Further study showed that miR-4644 exhibited higher stability and expression abundance than other commonly miRNA reference controls.
Results: 11 candidate miRNAs were obtained, and miR-4644 stood out among these miRNAs, and proved to be much more stable than other endogenous miRNAs. We also explored the availability of the miRNA combination, and compared the performance difference between combination and individuals, as well as the selectivity of miRNA references in the combinations. Furthermore, the stability of target genes was evaluated using geNorm, NormFinder, comparative ΔCq programs, and validated by database. Then, the expression levels of these genes in serum were analyzed by quantitative reverse transcription PCR (RT-qPCR) among four groups, including patients diagnosed with HCC, chronic hepatitis B (CHB), liver cirrhosis, and healthy subjects. Five commonly reference genes were chosen for further comparison and verification. Methods: Candidate miRNAs were selected by screening dataset GSE104310, as well as data statistics and analysis. In this study, we aim to identify a stable endogenous normalizer for analyzing circulating miRNAs. However, no general consensus on an ideal endogenous normalizer for circulating miRNAs quantification has been reached, so it will affect the accuracy of quantitative results. Particularly for hepatocellular carcinoma (HCC), detection of circulating miRNA biomarkers as a new diagnostic approach has been written into the latest Guidelines for Diagnosis and Treatment of Primary Liver Cancer in China (2019 edition). File import instruction Abstractīackground: Circulating microRNAs (miRNAs) have proved to be promising biomarkers for early diagnosis and therapeutic monitoring in cancers. Select the file that you have just downloaded and select import option Reference Manager (RIS).
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Available fromĬlick on Go to download the file. Identification of miR-4644 as a suitable endogenous normalizer for circulating miRNA quantification in hepatocellular carcinoma. Zhao J, Zhu XC, Wu XS, Wang L, Zhu CC, Yang K, Deng GQ, Wang A, Liu Y, Jia WD, Zhu L.
Wei-Dong Jia, Department of General Surgery, Anhui Provincial Hospital & the First Affiliated Hospital of USTC, Division of Life Science and Medicine, University of Science and Technology of China, No.17, Lujiang Road, Hefei 230001, China. ✉ Corresponding author: Ling Zhu, Center of Engineering Technology Research for Biomedical Optical Instrument, Anhui Institute of Optics and Fine Mechanics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, No.350, Shushanhu Road, Hef ei 230031, China. Department of General Surgery, Anhui Provincial Hospital & the First Affiliated Hospital of USTC, Division of Life Science and Medicine, University of Science and Technology of China, Hefei 230001, China. Science Island Branch of Graduate School, University of Science and Technology of China, Hefei 230026, China.ģ. Center of Engineering Technology Research for Biomedical Optical Instrument, Anhui Institute of Optics and Fine Mechanics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China.Ģ. Jun Zhao 1,2, Xin-Chao Zhu 1,2, Xiao-Song Wu 1,2, Lin Wang 1,2, Can-Can Zhu 1, Ke Yang 1, Guo-Qing Deng 1, An Wang 1, Yong Liu 1, Wei-Dong Jia 3, Ling Zhu 1ġ. Research Paper Identification of miR-4644 as a suitable endogenous normalizer for circulating miRNA quantification in hepatocellular carcinoma
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